| 人/猪/牛 胰岛素(INS)酶联免疫吸附试剂盒 |
| Catalog #: NE01I0004 |
| Sample Type: Biological samples |
Other Names
Human/Canine/Porcine Insulin ELISA Kit
INS
Research Area
Metabolism, Signal transduction, Cardiovascular, Neuroscience
Background
Insulin and the IGFs both belong to insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and are very similar in shape of an exaggerated, inverted letter G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110).
The removal of the C-peptide by proteolysis can enhance the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in clinical symptoms of hyperinsulinemia, obesity and type II diabetes.
|
Product Name |
INS ELISA |
|
Species |
Human,Canina,Porcine |
|
Product Size |
96/48 Tests |
|
Concentration |
0-500 pmol/L |
|
Sensitivity |
6.1pmol/L |
|
Principle |
Sandwich ELISA |
|
Sample Volume |
100 ul |
|
Assay Time |
3.5 hours |
|
Platform |
Microplate Reader |
|
Conjugate |
HRP |
|
Detection Method |
Colorimetric |
|
Storage |
2-8°C |
|
|
Four parameter Logisticcurve regression
Formular: y = (A - D) / [1 + (x/C)^B] + D
A = 5.98782 |
| Sample | Cross reactivity（%） |
|
TfR |
--- |
|
IL-8 |
--- |
| FGF | --- |
| IL-6 | --- |
| Dilution Ratio | Range % |
|
1:2 |
94-110 |
|
1:4 |
98-105 |
|
1:8 |
98-103 |
|
1:16 |
97-106 |
|
1:32 |
96-102 |
|
1:64 |
98-103 |